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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SYAP1 Monoclonal antibody

SYAP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E12A5

Cat no : CL488-68410

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SYAP1 Monoclonal ant (CL488-68410)

    IF Staining of HepG2 using CL488-68410

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SYAP1 antibody (CL488-68410, Clone: 2E12A5 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68410 targets SYAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SYAP1 fusion protein Ag9626 相同性解析による交差性が予測される生物種
Full Name synapse associated protein 1, SAP47 homolog (Drosophila)
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC014657
Gene symbol SYAP1
Gene ID (NCBI) 94056
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SYAP1 antibody CL488-68410Download protocol
Standard Protocols
Click here to view our Standard Protocols