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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SYK Monoclonal antibody

SYK Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C4A12

Cat no : CL594-66721

Synonyms

Tyrosine protein kinase SYK, spleen tyrosine kinase, p72-Syk, IMD82, EC:2.7.10.2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®594-conjugated SYK Monoclonal antibody (CL594-66721)

    FC experiment of K-562 using CL594-66721

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SYK (CL594-66721, Clone:4C4A12) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66721 targets SYK in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SYK fusion protein Ag17654 相同性解析による交差性が予測される生物種
Full Name spleen tyrosine kinase
Calculated molecular weight 635 aa, 72 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC001645
Gene symbol SYK
Gene ID (NCBI) 6850
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL594 SYK antibody CL594-66721Download protocol
Standard Protocols
Click here to view our Standard Protocols