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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNPO Recombinant antibody (80721-1-RR)

    WB analysis using 80721-1-RR (same clone as 80721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse brain using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse brain using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80721-1-RR

    Affinity and Kinetic Characterization of 80721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

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SYNPO Recombinant antibody, PBS Only

SYNPO Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80721-1-PBS
Clone No.4K5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Synaptopodin, snaptopodin, KIAA1029, 4K5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80721-1-PBS targets SYNPO in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYNPO fusion protein Ag31287 相同性解析による交差性が予測される生物種
Full Name synaptopodin
Calculated molecular weight 929 aa, 99 kDa
Observed molecular weight100 kDa
GenBank accession numberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_2918907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N3V7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)