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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNPO Recombinant antibody (80721-1-RR)

    WB analysis using 80721-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of human kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse brain using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of rat kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of rat kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse brain using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of mouse kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SYNPO Recombinant antibody (80721-1-RR)

    IHC staining of human kidney using 80721-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80721-1-RR

    Affinity and Kinetic Characterization of 80721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

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SYNPO Recombinant antibody

SYNPO Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 80721-1-RR
Clone No.4K5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

Synaptopodin, snaptopodin, KIAA1029, 4K5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:17800
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80721-1-RR targets SYNPO in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYNPO fusion protein Ag31287 相同性解析による交差性が予測される生物種
Full Name synaptopodin
Calculated molecular weight 929 aa, 99 kDa
Observed molecular weight100 kDa
GenBank accession numberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_2918907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N3V7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Protocols

Product Specific Protocols
WB protocol for SYNPO antibody 80721-1-RRDownload protocol
IHC protocol for SYNPO antibody 80721-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols