Synaptotagmin-14 Recombinant antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83923-1-RR (SYT14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

1x10^6 A431 cells were intracellularly stained with 0.25 ug Synaptotagmin-14 Recombinant antibody (83923-1-RR, Clone:240866C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.