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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    WB analysis using 83923-1-RR (same clone as 83923-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    WB analysis of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83923-1-RR (SYT14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    FC experiment of A431 using 83923-1-RR (same clone as 83923-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Synaptotagmin-14 Recombinant antibody (83923-1-RR, Clone:240866C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

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Synaptotagmin-14 Recombinant antibody, PBS Only

Synaptotagmin-14 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83923-1-PBS
Clone No.240866C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

SytXIV, SYT14, Synaptotagmin XIV, 240866C2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83923-1-PBS targets Synaptotagmin-14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synaptotagmin XIV
Calculated molecular weight 62 kDa
Observed molecular weight62 kDa
GenBank accession numberNM_153262
Gene Symbol Synaptotagmin-14
Gene ID (NCBI) 255928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NB59
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).