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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    WB analysis using 83923-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using SYT14 Recombinant antibody (83923-1-RR)

    WB analysis of mouse cerebellum using 83923-1-RR

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83923-1-RR (SYT14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    IHC staining of mouse cerebellum using 83923-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    IHC staining of mouse cerebellum using 83923-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using Synaptotagmin-14 Recombinant antibody (83923-1-RR)

    FC experiment of A431 using 83923-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Synaptotagmin-14 Recombinant antibody (83923-1-RR, Clone:240866C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Synaptotagmin-14 Recombinant antibody

Synaptotagmin-14 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83923-1-RR
Clone No.240866C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

SytXIV, SYT14, Synaptotagmin XIV, 240866C2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83923-1-RR targets Synaptotagmin-14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synaptotagmin XIV
Calculated molecular weight 62 kDa
Observed molecular weight62 kDa
GenBank accession numberNM_153262
Gene Symbol Synaptotagmin-14
Gene ID (NCBI) 255928
RRIDAB_3671503
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NB59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-14 antibody 83923-1-RRDownload protocol
IHC protocol for Synaptotagmin-14 antibody 83923-1-RRDownload protocol
FC protocol for Synaptotagmin-14 antibody 83923-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols