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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYTL2 Polyclonal antibody (12359-1-AP)

    WB analysis using 12359-1-AP (same clone as 12359-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12359-1-AP (SYTL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12359-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SYTL2 Polyclonal antibody (12359-1-AP)

    WB analysis of Jurkat using 12359-1-AP (same clone as 12359-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12359-1-AP (SYTL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12359-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SYTL2 Polyclonal antibody (12359-1-AP)

    IHC staining of human breast cancer using 12359-1-AP (same clone as 12359-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12359-1-AP (SYTL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12359-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SYTL2 Polyclonal antibody (12359-1-AP)

    IHC staining of human breast cancer using 12359-1-AP (same clone as 12359-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12359-1-AP (SYTL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12359-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SYTL2 Polyclonal antibody, PBS Only

SYTL2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12359-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12359-1-PBS targets SYTL2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYTL2 fusion protein Ag3023 相同性解析による交差性が予測される生物種
Full Name synaptotagmin-like 2
Calculated molecular weight 934 aa, 105 kDa
Observed molecular weight39 kDa, 100-105 kDa
GenBank accession numberBC015540
Gene Symbol SYTL2
Gene ID (NCBI) 54843
RRIDAB_11182492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCH5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Synaptotagmin-like 2 (SYTL2) is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. SYTL2 contains an N-terminal Slp homology domain (SHD), Rab-binding region, and C-terminal tandem C2 domains, C2A and C2B (PMID: 11327731). It interacts with RAB27A and plays a role in promoting docking of RAB27-bound organelles to the plasma membrane (PMID: 17052176). Alternative splicing of SYTL2 gene results in multiple transcript variants encoding distinct isoforms with calculated molecular weights ranging from 39 kDa to 142 kDa.