CoraLite®594-conjugated Sam50 Monoclonal antibody
Sam50 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Pig, Rat, Mouse
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2H6H9
Cat no : CL594-67425
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67425 targets Sam50 in IF/ICC applications and shows reactivity with Human, Pig, Rat, Mouse samples.
| Tested Reactivity | Human, Pig, Rat, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Sam50 fusion protein Ag15155 相同性解析による交差性が予測される生物種 |
| Full Name | sorting and assembly machinery component 50 homolog (S. cerevisiae) |
| Calculated molecular weight | 469 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC007830 |
| Gene symbol | Sam50 |
| Gene ID (NCBI) | 25813 |
| RRID | AB_2920133 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Sam50 antibody CL594-67425 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
