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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Septin 2 Polyclonal antibody

Septin 2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11397

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Septin 2 Polyclonal antibo (CL594-11397)

    IF Staining of HepG2 using CL594-11397

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Septin 2 antibody (CL594-11397) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated Septin 2 Polyclonal antibo (CL594-11397)

    FC experiment of MCF-7 using CL594-11397

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Septin 2 (CL594-11397) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11397 targets Septin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 2 fusion protein Ag1946 相同性解析による交差性が予測される生物種
Full Name septin 2
Calculated molecular weight 41 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC014455
Gene symbol Septin 2
Gene ID (NCBI) 4735
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.

What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?

The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).

What is the subcellular localization of SEPT2?

SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.

What is the tissue expression pattern of SEPT2?

While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).


Protocols

Product Specific Protocols
IF protocol for CL594 Septin 2 antibody CL594-11397Download protocol
Standard Protocols
Click here to view our Standard Protocols