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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis using 68075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis of LNCaP using 68075-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis using 68075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using Septin 5 Monoclonal antibody (68075-1-Ig)

    FC experiment of Jurkat using 68075-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Septin 5 Monoclonal antibody (68075-1-Ig, Clone:1E2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Septin 5 Monoclonal antibody

Septin 5 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68075-1-Ig
Clone No.1E2A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), ELISA

Synonyms

SEPT5, Cell division control-related protein 1, CDCrel-1, CDCREL1, CDCREL 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig brain tissue, LNCaP cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68075-1-Ig targets Septin 5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 5 fusion protein Ag2205 相同性解析による交差性が予測される生物種
Full Name septin 5
Calculated molecular weight 369 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC025261
Gene Symbol Septin 5
Gene ID (NCBI) 5413
RRIDAB_2918813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99719
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

Protocols

Product Specific Protocols
WB protocol for Septin 5 antibody 68075-1-IgDownload protocol
FC protocol for Septin 5 antibody 68075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols