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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Septin 5 Monoclonal antibody

Septin 5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2A3

Cat no : 68075-1-Ig

Synonyms

CDCREL, CDCREL 1, CDCREL1, H5, Peanut like protein 1, PNUTL1, SEPT5, septin 5, septin5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis using 68075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis of LNCaP using 68075-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis using 68075-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inpig brain tissue, LNCaP cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68075-1-Ig targets Septin 5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 5 fusion protein Ag2205 相同性解析による交差性が予測される生物種
Full Name septin 5
Calculated molecular weight 369 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC025261
Gene symbol Septin 5
Gene ID (NCBI) 5413
RRIDAB_2918813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

Protocols

Product Specific Protocols
WB protocol for Septin 5 antibody 68075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols