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Host
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Septin 5 Monoclonal antibody, PBS Only

Septin 5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2A3

Cat no : 68075-1-PBS

Synonyms

CDCREL, CDCREL 1, CDCREL1, H5, Peanut like protein 1, PNUTL1, SEPT5, septin 5, septin5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis using 68075-1-Ig (same clone as 68075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68075-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis of LNCaP using 68075-1-Ig (same clone as 68075-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68075-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Septin 5 Monoclonal antibody (68075-1-Ig)

    WB analysis using 68075-1-Ig (same clone as 68075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68075-1-Ig (Septin 5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68075-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68075-1-PBS targets Septin 5 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 5 fusion protein Ag2205 相同性解析による交差性が予測される生物種
Full Name septin 5
Calculated molecular weight 369 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC025261
Gene symbol Septin 5
Gene ID (NCBI) 5413
RRIDAB_2918813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.