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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Septin 7 Monoclonal antibody

Septin 7 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G12D1

Cat no : CL488-66542

Synonyms

septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated Septin 7 Monoclonal (CL488-66542)

    IF Staining of MDCK using CL488-66542

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Septin 7 antibody (CL488-66542, Clone: 1G12D1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66542 targets Septin 7 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 7 fusion protein Ag4309 相同性解析による交差性が予測される生物種
Full Name septin 7
Calculated molecular weight 51 kDa
Observed molecular weight 48-51 kDa
GenBank accession numberBC025987
Gene symbol Septin 7
Gene ID (NCBI) 989
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Septin 7 antibody CL488-66542Download protocol
Standard Protocols
Click here to view our Standard Protocols