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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

    WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    WB analysis of pig liver using 66297-1-Ig (same clone as 66297-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    WB analysis of L02 using 66297-1-Ig (same clone as 66297-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    WB analysis of HEK-293 using 66297-1-Ig (same clone as 66297-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    WB analysis of ROS1728 using 66297-1-Ig (same clone as 66297-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Sestrin 2 Monoclonal antibody (66297-1-Ig)

    IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Sestrin 2 Monoclonal antibody, PBS Only

Sestrin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66297-1-PBS
Clone No.1A1A4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66297-1-PBS targets Sestrin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag20638 相同性解析による交差性が予測される生物種
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC013304
Gene Symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_2881680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP58004
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).