☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 10795-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration:  150 μL, 750 ug/ml
20 μL, 750 ug/ml
150 μL, 750 ug/ml

¥67,000/ 150 μL

本製品の国内在庫をすぐに見る(表示に数秒かかります)

【全製品対象☆】小さなギフトがついてくる!春の特典キャンペーン開催中

他社製品の代替品をお探しですか?『抗体代替品/同等品検索システム』でお調べいただけます


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, human liver tissue, human brain tissue, NIH/3T3 cells, rat liver tissue, HeLa cells, K-562 cells, mouse kidney tissue, A2780 cells, RAW 264.7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10795-1-AP targets Sestrin 2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag1247 相同性解析による交差性が予測される生物種
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC013304
Gene Symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_2185480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IHC protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IF protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IP protocol for Sestrin 2 antibody 10795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An
mouseWB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
humanWB

Nature

Stem cell function and stress response are controlled by protein synthesis.

Authors - Sandra Blanco
mouseWB

Science

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Authors - Andrew L Cangelosi
humanWB

Nature

SZT2 dictates GATOR control of mTORC1 signalling.

Authors - Min Peng
mouseWB

Cell

Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.

Authors - Min Peng
  • KO Validated
Loading...