Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 10795-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10795-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10795-1-AP
WB result of Sestrin antibody (10795-1-AP, 1:2000) with si-Control and si-Sestrin transfected HEK293 cells.
WB analysis of human brain using 10795-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10795-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10795-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of human liver using 10795-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10795-1-AP (Sestrin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 10795-1-AP
IP result of anti-Sestrin 2 (IP:10795-1-AP, 3ug; Detection:10795-1-AP 1:1000) with K-562 cells lysate 4000ug.
IHC staining of human breast cancer using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary tumor using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon cancer using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10795-1-AP (Sestrin 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10795-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10795-1-AP (Sestrin 2 antibody) at dilution of 1:50 (under 25x lens).
IF Staining of mouse kidney using 10795-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Sestrin 2 antibody (10795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10795-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (10795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10795-1-AP

Publications(217)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (217)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, human liver tissue, human brain tissue, NIH/3T3 cells, rat liver tissue, HeLa cells, K-562 cells, mouse kidney tissue, A2780 cells, RAW 264.7 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 57 publications below
WB	See 196 publications below
IHC	See 21 publications below
IF	See 29 publications below
IP	See 12 publications below
FC	See 2 publications below
CoIP	See 3 publications below

Product Information

10795-1-AP targets Sestrin 2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1247 Product name: Recombinant human Sestrin2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-480 aa of BC013304 Sequence: HMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT 相同性解析による交差性が予測される生物種
Full Name	sestrin 2
Calculated molecular weight	54 kDa
Observed molecular weight	54-60 kDa
GenBank accession number	BC013304
Gene Symbol	SESN2/Sestrin 2
Gene ID (NCBI)	83667
RRID	AB_2185480
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P58004
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Protocols

Product Specific Protocols
IF protocol for Sestrin 2 antibody 10795-1-AP	Download protocol
IHC protocol for Sestrin 2 antibody 10795-1-AP	Download protocol
IP protocol for Sestrin 2 antibody 10795-1-AP	Download protocol
WB protocol for Sestrin 2 antibody 10795-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Systematic quantitative analysis of ribosome inventory during nutrient stress. Authors - Heeseon An View Article
mouse	WB	Nature SAR1B senses leucine levels to regulate mTORC1 signalling. Authors - Jie Chen View Article
human	WB	Nature Stem cell function and stress response are controlled by protein synthesis. Authors - Sandra Blanco View Article
mouse	WB	Science Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine. Authors - Andrew L Cangelosi View Article
human	WB	Nature SZT2 dictates GATOR control of mTORC1 signalling. Authors - Min Peng View Article
mouse	WB	Cell Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling. Authors - Min Peng KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10795-1-AP

WB analysis using 10795-1-AP

WB analysis of HEK-293 using 10795-1-AP

WB analysis of human brain using 10795-1-AP

WB analysis of NIH/3T3 using 10795-1-AP

WB analysis of rat liver using 10795-1-AP

WB analysis of human liver using 10795-1-AP

IP experiment of K-562 using 10795-1-AP

IHC staining of human breast cancer using 10795-1-AP

IHC staining of human breast cancer using 10795-1-AP

IHC staining of human ovary tumor using 10795-1-AP

IHC staining of human ovary tumor using 10795-1-AP

IHC staining of human breast cancer using 10795-1-AP

IHC staining of human breast cancer using 10795-1-AP

IHC staining of human colon cancer using 10795-1-AP

IHC staining of human colon cancer using 10795-1-AP

IHC staining of human ovary tumor using 10795-1-AP

IF Staining of mouse kidney using 10795-1-AP

IF Staining of HeLa using 10795-1-AP

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Systematic quantitative analysis of ribosome inventory during nutrient stress.

SAR1B senses leucine levels to regulate mTORC1 signalling.

Stem cell function and stress response are controlled by protein synthesis.

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

SZT2 dictates GATOR control of mTORC1 signalling.

Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.