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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10795

Synonyms

DKFZp761M0212, DKFZp761M02121, HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Sestrin 2 Polyclonal (CL488-10795)

    IF Staining of HeLa using CL488-10795

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Sestrin 2 antibody (CL488-10795) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10795 targets Sestrin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag1247 相同性解析による交差性が予測される生物種
Full Name sestrin 2
Calculated molecular weight 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC013304
Gene symbol SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_2918996
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Sestrin 2 antibody CL488-10795Download protocol
Standard Protocols
Click here to view our Standard Protocols