Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 12369-1-AP (same clone as 12369-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12369-1-AP (same clone as 12369-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IP experiment of rat brain using 12369-1-AP (same clone as 12369-1-PBS)
IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 12369-1-AP (same clone as 12369-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sortilin antibody (12369-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
FC experiment of Neuro-2a using 12369-1-AP (same clone as 12369-1-PBS)
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.
×
1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Sortilin Polyclonal antibody, PBS Only

Sortilin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Cat No. 12369-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12369-1-PBS targets Sortilin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Sortilin fusion protein Ag2997 相同性解析による交差性が予測される生物種
Full Name	sortilin 1
Calculated molecular weight	831 aa, 92 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	BC023542
Gene Symbol	Sortilin
Gene ID (NCBI)	6272
ENSEMBL Gene ID	ENSG00000134243
RRID	AB_2302242
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99523
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12369-1-AP (same clone as 12369-1-PBS)

WB analysis of human brain using 12369-1-AP (same clone as 12369-1-PBS)

IP experiment of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)