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  • Featured Product
  • KD/KO Validated

Sortilin Polyclonal antibody, PBS Only

Sortilin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12369-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Sortilin Polyclonal antibody (12369-1-AP)

    WB analysis using 12369-1-AP (same clone as 12369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    WB analysis of human brain using 12369-1-AP (same clone as 12369-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12369-1-AP (Sortilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IP experiment of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

    IP result of anti-Sortilin (IP:12369-1-AP, 4ug; Detection:12369-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IHC staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IHC staining of human brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IHC staining of human brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12369-1-AP (Sortilin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IHC staining of rat brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12369-1-AP (Sortilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Sortilin Polyclonal antibody (12369-1-AP)

    IF Staining of mouse brain using 12369-1-AP (same clone as 12369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (12369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Neuro-2a cells using Sortilin Polyclonal antibody (12369-1-AP)

    FC experiment of Neuro-2a using 12369-1-AP (same clone as 12369-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human Sortilin (12369-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12369-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12369-1-PBS targets Sortilin in WB, IHC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sortilin fusion protein Ag2997 相同性解析による交差性が予測される生物種
Full Name sortilin 1
Calculated molecular weight 831 aa, 92 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC023542
Gene symbol Sortilin
Gene ID (NCBI) 6272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.