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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Spermidine synthase (CL488-68548)

    IF Staining of HepG2 using CL488-68548

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Spermidine synthase antibody (CL488-68548, Clone: 2F4E10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CoraLite® Plus 488-conjugated Spermidine synthase (CL488-68548)

    IF Staining of RAW 264.7 using CL488-68548

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 Spermidine synthase antibody (CL488-68548, Clone: 2F4E10 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated Spermidine synthase Monoclonal antibody

Spermidine synthase Monoclonal Antibody for IF/ICC
Cat No. CL488-68548
Clone No.2F4E10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

SRM, SPS1, SPDSY, PAPT, EC:2.5.1.16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inRAW 264.7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68548 targets Spermidine synthase in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Spermidine synthase fusion protein Ag33919 相同性解析による交差性が予測される生物種
Full Name spermidine synthase
Calculated molecular weight 302 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC000309
Gene Symbol Spermidine synthase
Gene ID (NCBI) 6723
RRIDAB_3673024
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19623
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Spermidine synthase antibody CL488-68548Download protocol
Standard Protocols
Click here to view our Standard Protocols