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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Stanniocalcin 2 Monoclonal (CL594-60063)

    IF Staining of HeLa using CL594-60063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60063 (Stanniocalcin 2 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Stanniocalcin 2 Monoclonal (CL594-60063)

    FC experiment of HeLa using CL594-60063

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Stanniocalcin 2 (CL594-60063, Clone:4H1E7) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60063
Clone No.4H1E7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60063 targets Stanniocalcin 2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Stanniocalcin 2 fusion protein Ag0359 相同性解析による交差性が予測される生物種
Full Name stanniocalcin 2
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000658
Gene Symbol Stanniocalcin 2
Gene ID (NCBI) 8614
RRIDAB_2883414
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO76061
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Protocols

Product Specific Protocols
IF protocol for CL594 Stanniocalcin 2 antibody CL594-60063Download protocol
Standard Protocols
Click here to view our Standard Protocols