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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Stathmin 1 Recombinant antibody

Stathmin 1 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H6

Cat no : CL488-82559

Synonyms

STMN1, Stathmin, OP18, Leukemia-associated phosphoprotein p18, LAP18

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Stathmin 1 Recombina (CL488-82559)

    IF Staining of HeLa using CL488-82559

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Stathmin 1 antibody (CL488-82559, Clone: 2H6 ) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Stathmin 1 Recombina (CL488-82559)

    IF Staining of HeLa using CL488-82559

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Stathmin 1 antibody (CL488-82559, Clone: 2H6 ) at dilution of 1:400, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Stathmin 1 Recombina (CL488-82559)

    FC experiment of HeLa using CL488-82559

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Stathmin 1 Recombinant antibody (CL488-82559, Clone:2H6)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82559 targets Stathmin 1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Stathmin 1 fusion protein Ag1633 相同性解析による交差性が予測される生物種
Full Name stathmin 1/oncoprotein 18
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC014353
Gene symbol Stathmin 1
Gene ID (NCBI) 3925
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Stathmin 1 antibody CL488-82559Download protocol
FC protocol for CL Plus 488 Stathmin 1 antibody CL488-82559Download protocol
Standard Protocols
Click here to view our Standard Protocols