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  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated Surfactant Protein A (CL488-11850)

    FC experiment of A549 using CL488-11850

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Surfactant Protein A (CL488-11850)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated Surfactant Protein A Polyclonal antibody

Surfactant Protein A Polyclonal Antibody for FC (Intra)
Cat No. CL488-11850

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Alveolar proteinosis protein, COLEC4, Collectin 4, FLJ51913, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SFTPA2, SP A, SP A1, Surfactant Protein A, surfactant protein A1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inA549 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11850 targets Surfactant Protein A in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Surfactant Protein A fusion protein Ag2417 相同性解析による交差性が予測される生物種
Full Name surfactant protein A1
Calculated molecular weight 248 aa, 26 kDa
GenBank accession numberBC026229
Gene Symbol Surfactant Protein A
Gene ID (NCBI) 653509
RRIDAB_3672515
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWL2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Surfactant Protein A antibody CL488-11850Download protocol
Standard Protocols
Click here to view our Standard Protocols