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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptophysin Recombinant antibody

Synaptophysin Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

2H14

Cat no : 82900-1-RR

Synonyms

SYP, SYN, MRX96, 2H14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptophysin Recombinant antibody (82900-1-RR)

    WB analysis using 82900-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82900-1-RR (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Synaptophysin Recombinant antibody (82900-1-RR)

    IP experiment of mouse brain using 82900-1-RR

    IP result of anti-Synaptophysin (IP:82900-1-RR, 4ug; Detection:82900-1-RR 1:5000) with mouse brain tissue lysate 1800 ug.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Synaptophysin Recombinant antibody (82900-1-RR)

    IHC staining of mouse pancreas using 82900-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Synaptophysin Recombinant antibody (82900-1-RR)

    IHC staining of mouse pancreas using 82900-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Synaptophysin Recombinant antibody (82900-1-RR)

    IF Staining of PC-12 using 82900-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Synaptophysin Recombinant antibody (82900-1-RR)

    IF Staining of U2OS using 82900-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A549 cells using Synaptophysin Recombinant antibody (82900-1-RR)

    FC experiment of A549 using 82900-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Synaptophysin Recombinant antibody (82900-1-RR, Clone:2H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82900-1-RR

    Affinity and Kinetic Characterization of 82900-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82900-1-RR against Human Synaptophysin were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inPC-12 cells, human cerebellum tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, U2OS cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82900-1-RR targets Synaptophysin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781 相同性解析による交差性が予測される生物種
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC064550
Gene symbol Synaptophysin
Gene ID (NCBI) 6855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
WB protocol for Synaptophysin antibody 82900-1-RRDownload protocol
IHC protocol for Synaptophysin antibody 82900-1-RRDownload protocol
IF protocol for Synaptophysin antibody 82900-1-RRDownload protocol
IP protocol for Synaptophysin antibody 82900-1-RRDownload protocol
FC protocol for Synaptophysin antibody 82900-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols