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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Synaptophysin Recombinant antibody

Synaptophysin Recombinant Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H14

Cat no : CL488-82900

Synonyms

SYP, SYN, MRX96, 2H14

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Recomb (CL488-82900)

    IF Staining of mouse brain using CL488-82900

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-82900, Clone: 2H14 ) at dilution of 1:200, Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CoraLite® Plus 488-conjugated Synaptophysin Recomb (CL488-82900)

    IF Staining of PC-12 using CL488-82900

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 Synaptophysin antibody (CL488-82900, Clone: 2H14 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82900 targets Synaptophysin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781 相同性解析による交差性が予測される生物種
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight38-40 kDa
GenBank accession numberBC064550
Gene symbol Synaptophysin
Gene ID (NCBI) 6855
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Synaptophysin antibody CL488-82900Download protocol
Standard Protocols
Click here to view our Standard Protocols