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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-17785

Synonyms

SYP, SYN, MRX96

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse brain using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:1000.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    IF Staining of mouse brain using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 647-conjugated Synaptophysin Polycl (CL647-17785)

    FC experiment of PC-12 using CL647-17785

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Synaptophysin (CL647-17785) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse pancreas tissue, mouse brain tissue
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-17785 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781 相同性解析による交差性が予測される生物種
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC064550
Gene symbol Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2920235
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Synaptophysin antibody CL647-17785Download protocol
Standard Protocols
Click here to view our Standard Protocols