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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4E12C4

Cat no : CL647-67864

Synonyms

synaptophysin, SYP

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Monocl (CL647-67864)

    IF Staining of mouse brain using CL647-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Monocl (CL647-67864)

    IF Staining of mouse brain using CL647-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Synaptophysin Monocl (CL647-67864)

    IF Staining of mouse brain using CL647-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-67864, Clone: 4E12C4 ) at dilution of 1:2000.

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Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67864 targets Synaptophysin in IF-P applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11803 相同性解析による交差性が予測される生物種
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
GenBank accession numberBC064550
Gene symbol Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2920318
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Synaptophysin antibody CL647-67864Download protocol
Standard Protocols
Click here to view our Standard Protocols