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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-1 Monoclonal antibody

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H1H2

Cat no : 68043-1-Ig

Synonyms

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    WB analysis using 68043-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    WB analysis of rat cerebellum using 68043-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    WB analysis of mouse cerebellum using 68043-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse brain using 68043-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse brain using 68043-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse cerebellum using 68043-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse cerebellum using 68043-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, rat cerebellum tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rabbit cerebellum tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68043-1-Ig targets Synaptotagmin-1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptotagmin-1 fusion protein Ag6071 相同性解析による交差性が予測される生物種
Full Name synaptotagmin I
Calculated molecular weight 48 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC058917
Gene symbol Synaptotagmin-1
Gene ID (NCBI) 6857
RRIDAB_2918785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-1 antibody 68043-1-IgDownload protocol
IHC protocol for Synaptotagmin-1 antibody 68043-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols