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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Synaptotagmin-1 Monoclonal antibody

Synaptotagmin-1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H1H2

Cat no : CL488-68043

Synonyms

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

Validation Data Gallery

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  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated Synaptotagmin-1 Mono (CL488-68043)

    FC experiment of SH-SY5Y using CL488-68043

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated Synaptotagmin-1 Monoclonal antibody (CL488-68043, Clone:1H1H2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68043 targets Synaptotagmin-1 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptotagmin-1 fusion protein Ag6071 相同性解析による交差性が予測される生物種
Full Name synaptotagmin I
Calculated molecular weight 48 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC058917
Gene symbol Synaptotagmin-1
Gene ID (NCBI) 6857
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Synaptotagmin-1 antibody CL488-68043Download protocol
Standard Protocols
Click here to view our Standard Protocols