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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntaxin 10 Monoclonal antibody

Syntaxin 10 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1C3

Cat no : 66821-1-Ig

Synonyms

hsyn10, STX10, SYN10, syntaxin 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 10 Monoclonal antibody (66821-1-Ig)

    WB analysis using 66821-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Syntaxin 10 Monoclonal antibody (66821-1-Ig)

    IF Staining of HeLa using 66821-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, T-47D cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66821-1-Ig targets Syntaxin 10 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 10 fusion protein Ag27861 相同性解析による交差性が予測される生物種
Full Name syntaxin 10
Calculated molecular weight 28 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017237
Gene symbol Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2882164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 10 antibody 66821-1-IgDownload protocol
IF protocol for Syntaxin 10 antibody 66821-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols