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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntaxin 10 Monoclonal antibody, PBS Only (Detector)

Syntaxin 10 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1C3

Cat no : 66821-1-PBS

Synonyms

Syntaxin-10, Syn10, STX10, 1B1C3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 10 Monoclonal antibody (66821-1-Ig)

    WB analysis using 66821-1-Ig (same clone as 66821-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66821-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Syntaxin 10 Monoclonal antibody (66821-1-Ig)

    IF Staining of HeLa using 66821-1-Ig (same clone as 66821-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66821-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51170-3

    Cytometric bead array standard curve of MP51170-3

    Cytometric bead array standard curve of MP51170-3, STX10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66821-5-PBS. Detection antibody: 66821-1-PBS. Standard:Ag27861. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66821-1-PBS targets Syntaxin 10 as part of a matched antibody pair:

MP51170-3: 66821-5-PBS capture and 66821-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 10 fusion protein Ag27861 相同性解析による交差性が予測される生物種
Full Name syntaxin 10
Calculated molecular weight 28 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017237
Gene symbol Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2882164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.