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Species Reactivity

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  • Featured Product
  • KD/KO Validated

Syntenin-1 Polyclonal antibody, PBS Only

Syntenin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22399-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntenin-1 Polyclonal antibody (22399-1-AP)

    WB analysis using 22399-1-AP (same clone as 22399-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    WB analysis of human placenta using 22399-1-AP (same clone as 22399-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Syntenin-1 Polyclonal antibody (22399-1-AP)

    WB analysis using 22399-1-AP (same clone as 22399-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IP experiment of HeLa using 22399-1-AP (same clone as 22399-1-PBS)

    IP result of anti-Syntenin-1 (IP:22399-1-AP, 3ug; Detection:22399-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IHC staining of human liver cancer using 22399-1-AP (same clone as 22399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IHC staining of human liver cancer using 22399-1-AP (same clone as 22399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IHC staining of human heart using 22399-1-AP (same clone as 22399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IHC staining of human heart using 22399-1-AP (same clone as 22399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IHC staining of mouse brain using 22399-1-AP (same clone as 22399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IHC staining of mouse brain using 22399-1-AP (same clone as 22399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Syntenin-1 Polyclonal antibody (22399-1-AP)

    IF Staining of HeLa using 22399-1-AP (same clone as 22399-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Syntenin-1 antibody (22399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22399-1-PBS targets Syntenin-1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntenin-1 fusion protein Ag18078 相同性解析による交差性が予測される生物種
Full Name syndecan binding protein (syntenin)
Calculated molecular weight 298 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC113674
Gene symbol Syntenin-1
Gene ID (NCBI) 6386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.