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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TADA1L Recombinant antibody (83641-4-RR)

    WB analysis using 83641-4-RR (same clone as 83641-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using TADA1L Recombinant antibody (83641-4-RR)

    WB analysis of human testis using 83641-4-RR (same clone as 83641-4-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TADA1L Recombinant antibody (83641-4-RR)

    IF Staining of MCF-7 using 83641-4-RR (same clone as 83641-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TADA1L antibody (83641-4-RR, Clone: 240637F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using TADA1L Recombinant antibody (83641-4-RR)

    FC experiment of HepG2 using 83641-4-RR (same clone as 83641-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83641-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00624-1

    Cytometric bead array standard curve of MP00624-1

    Cytometric bead array standard curve of MP00624-1, TADA1L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83641-4-PBS. Detection antibody: 83641-5-PBS. Standard: Ag14118. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83641-4-RR

    Affinity and Kinetic Characterization of 83641-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

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TADA1L Recombinant antibody, PBS Only (Capture)

TADA1L Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83641-4-PBS
Clone No.240637F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4, KIAA0764

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83641-4-PBS targets TADA1L as part of a matched antibody pair:

MP00624-1: 83641-4-PBS capture and 83641-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TADA1L fusion protein Ag14118 相同性解析による交差性が予測される生物種
Full Name transcriptional adaptor 1 (HFI1 homolog, yeast)-like
Calculated molecular weight 335 aa, 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC015401
Gene Symbol TADA1L
Gene ID (NCBI) 117143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96BN2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.