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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TADA1L Recombinant antibody (83641-4-RR)

    WB analysis using 83641-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TADA1L Recombinant antibody (83641-4-RR)

    WB analysis of human testis using 83641-4-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TADA1L Recombinant antibody (83641-4-RR)

    IF Staining of MCF-7 using 83641-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TADA1L antibody (83641-4-RR, Clone: 240637F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using TADA1L Recombinant antibody (83641-4-RR)

    FC experiment of HepG2 using 83641-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83641-4-RR

    Affinity and Kinetic Characterization of 83641-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

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TADA1L Recombinant antibody

TADA1L Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83641-4-RR
Clone No.240637F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

STAF42, SPT3-associated factor 42, SPT3 associated factor 42, RP1 9E21.4, KIAA0764

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, human testis tissue, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83641-4-RR targets TADA1L in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TADA1L fusion protein Ag14118 相同性解析による交差性が予測される生物種
Full Name transcriptional adaptor 1 (HFI1 homolog, yeast)-like
Calculated molecular weight 335 aa, 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC015401
Gene Symbol TADA1L
Gene ID (NCBI) 117143
RRIDAB_3671252
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96BN2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.

Protocols

Product Specific Protocols
WB protocol for TADA1L antibody 83641-4-RRDownload protocol
IF protocol for TADA1L antibody 83641-4-RRDownload protocol
FC protocol for TADA1L antibody 83641-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols