CoraLite® Plus 488-conjugated TAF12 Monoclonal antibody
TAF12 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4E12D2
Cat no : CL488-67621
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67621 targets TAF12 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TAF12 fusion protein Ag16597 相同性解析による交差性が予測される生物種 |
| Full Name | TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC011986 |
| Gene symbol | TAF12 |
| Gene ID (NCBI) | 6883 |
| RRID | AB_3084365 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 TAF12 antibody CL488-67621 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
