• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

transgelin/SM22 Recombinant antibody, PBS Only

transgelin/SM22 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240864A11

Cat no : 83922-2-PBS

Synonyms

SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using transgelin/SM22 Recombinant antibody (83922-2-RR)

    WB analysis using 83922-2-RR (same clone as 83922-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using transgelin/SM22 Recombinant antibody (83922-2-RR)

    IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using transgelin/SM22 Recombinant antibody (83922-2-RR)

    IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83922-2-RR

    Affinity and Kinetic Characterization of 83922-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83922-2-RR against Human transgelin/SM22 were performed. The affinity constant is 14.2 nM.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83922-2-PBS is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC004927
Gene symbol transgelin/SM22
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.