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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

transgelin/SM22 Recombinant antibody

transgelin/SM22 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240864A11

Cat no : 83922-2-RR

Synonyms

SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using transgelin/SM22 Recombinant antibody (83922-2-RR)

    WB analysis using 83922-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using transgelin/SM22 Recombinant antibody (83922-2-RR)

    IHC staining of mouse heart using 83922-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using transgelin/SM22 Recombinant antibody (83922-2-RR)

    IHC staining of mouse heart using 83922-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83922-2-RR

    Affinity and Kinetic Characterization of 83922-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83922-2-RR against Human transgelin/SM22 were performed. The affinity constant is 14.2 nM.

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Tested Applications

Positive WB detected inmouse colon tissue, rat colon tissue, mouse stomach tissue, rat stomach tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83922-2-RR is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764 相同性解析による交差性が予測される生物種
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC004927
Gene symbol transgelin/SM22
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.

Protocols

Product Specific Protocols
WB protocol for transgelin/SM22 antibody 83922-2-RRDownload protocol
IHC protocol for transgelin/SM22 antibody 83922-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols