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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TAK1 Monoclonal anti (CL488-67707)

    IF Staining of HepG2 using CL488-67707

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 TAK1 antibody (CL488-67707, Clone: 1A5B2 ) at dilution of 1:200, CL594-phalloidin (red).

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CoraLite® Plus 488-conjugated TAK1 Monoclonal antibody

TAK1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67707
Clone No.1A5B2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67707 targets TAK1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAK1 fusion protein Ag21286 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated molecular weight 579 aa, 64 kDa
Observed molecular weight67-70 kDa
GenBank accession numberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
RRIDAB_3084374
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43318
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TAK1 antibody CL488-67707Download protocol
Standard Protocols
Click here to view our Standard Protocols