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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAPBPL Monoclonal antibody

TAPBPL Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D12C2

Cat no : 68577-1-Ig

Synonyms

TAP-binding protein-like, Tapasin-related protein, TAPASIN-R, Tapasin-like, TAPASIN R

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAPBPL Monoclonal antibody (68577-1-Ig)

    WB analysis using 68577-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using TAPBPL Monoclonal antibody (68577-1-Ig)

    WB analysis using 68577-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using TAPBPL Monoclonal antibody (68577-1-Ig)

    FC experiment of A431 using 68577-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.4 ug TAPBPL Monoclonal antibody (68577-1-Ig, Clone: 1D12C2) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inSCaBER cells, MCF-7 cells, HT-1376 cells, A431 cells, U-87 MG cells, Jurkat cells, human placenta tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68577-1-Ig targets TAPBPL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAPBPL fusion protein Ag14371 相同性解析による交差性が予測される生物種
Full Name TAP binding protein-like
Calculated molecular weight 468 aa, 50 kDa
Observed molecular weight55 kDa
GenBank accession numberBC015017
Gene symbol TAPBPL
Gene ID (NCBI) 55080
RRIDAB_3085277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAPBPL, also named as TAPBP-R and TAPASIN-R, is a member of the variable-constant Ig superfamily that links MHC class I molecules to the transporter associated with antigen processing in the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for TAPBPL antibody 68577-1-IgDownload protocol
FC protocol for TAPBPL antibody 68577-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols