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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAPBPL Monoclonal antibody (68577-1-Ig)

    WB analysis using 68577-1-Ig (same clone as 68577-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68577-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TAPBPL Monoclonal antibody (68577-1-Ig)

    WB analysis using 68577-1-Ig (same clone as 68577-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68577-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using TAPBPL Monoclonal antibody (68577-1-Ig)

    FC experiment of A431 using 68577-1-Ig (same clone as 68577-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.4 ug TAPBPL Monoclonal antibody (68577-1-Ig, Clone: 1D12C2) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68577-1-PBS in a different storage buffer formulation.

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TAPBPL Monoclonal antibody, PBS Only

TAPBPL Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68577-1-PBS
Clone No.1D12C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Synonyms

TAP-binding protein-like, Tapasin-related protein, TAPASIN-R, Tapasin-like, TAPASIN R

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68577-1-PBS targets TAPBPL in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAPBPL fusion protein Ag14371 相同性解析による交差性が予測される生物種
Full Name TAP binding protein-like
Calculated molecular weight 468 aa, 50 kDa
Observed molecular weight55 kDa
GenBank accession numberBC015017
Gene Symbol TAPBPL
Gene ID (NCBI) 55080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BX59
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TAPBPL, also named as TAPBP-R and TAPASIN-R, is a member of the variable-constant Ig superfamily that links MHC class I molecules to the transporter associated with antigen processing in the endoplasmic reticulum.