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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis using 67828-1-Ig (same clone as 67828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis of HSC-T6 using 67828-1-Ig (same clone as 67828-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis of 4T1 using 67828-1-Ig (same clone as 67828-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis of PC-12 using 67828-1-Ig (same clone as 67828-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TARS Monoclonal antibody (67828-1-Ig)

    IF Staining of HeLa using 67828-1-Ig (same clone as 67828-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

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TARS Monoclonal antibody, PBS Only

TARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67828-1-PBS
Clone No.1A11F10

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67828-1-PBS targets TARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TARS fusion protein Ag6614 相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase
Calculated molecular weight 83 kDa
Observed molecular weight 83-87 kDa
GenBank accession numberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2918590
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26639
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.