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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TARS Monoclonal antibody

TARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11F10

Cat no : 67828-1-Ig

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis using 67828-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis of HSC-T6 using 67828-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis of 4T1 using 67828-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using TARS Monoclonal antibody (67828-1-Ig)

    WB analysis of PC-12 using 67828-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TARS Monoclonal antibody (67828-1-Ig)

    IF Staining of HeLa using 67828-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, PC-12 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67828-1-Ig targets TARS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TARS fusion protein Ag6614 相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase
Calculated molecular weight 83 kDa
Observed molecular weight 83-87 kDa
GenBank accession numberBC000517
Gene symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2918590
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Protocols

Product Specific Protocols
WB protocol for TARS antibody 67828-1-IgDownload protocol
IF protocol for TARS antibody 67828-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
View Article