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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TARS Monoclonal antibody (CL594-67828)

    IF Staining of HeLa using CL594-67828

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 TARS antibody (CL594-67828, Clone: 1A11F10 ) at dilution of 1:200.

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CoraLite®594-conjugated TARS Monoclonal antibody

TARS Monoclonal Antibody for IF/ICC
Cat No. CL594-67828
Clone No.1A11F10

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67828 targets TARS in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TARS fusion protein Ag6614 相同性解析による交差性が予測される生物種
Full Name threonyl-tRNA synthetase
Calculated molecular weight 83 kDa
Observed molecular weight 83-87 kDa
GenBank accession numberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2923926
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26639
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Protocols

Product Specific Protocols
IF protocol for CL594 TARS antibody CL594-67828Download protocol
Standard Protocols
Click here to view our Standard Protocols