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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated WWTR1 Monoclonal antibody

WWTR1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A12A10

Cat no : CL488-66500

Synonyms

TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, 2A12A10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated WWTR1 Monoclonal ant (CL488-66500)

    WB analysis using CL488-66500

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66500 (WWTR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated WWTR1 Monoclonal ant (CL488-66500)

    WB analysis using CL488-66500

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66500 (WWTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66500 targets WWTR1 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WWTR1 fusion protein Ag13330 相同性解析による交差性が予測される生物種
Full Name WW domain containing transcription regulator 1
Calculated molecular weight 44 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC014052
Gene symbol WWTR1
Gene ID (NCBI) 25937
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 WWTR1 antibody CL488-66500Download protocol
Standard Protocols
Click here to view our Standard Protocols