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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 594-conjugated WWTR1 Monoclonal ant (CL594-66500)

    IF Staining of human liver cancer using CL594-66500

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 594 TAZ antibody (CL594-66500, Clone: 2A12A10 ) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated WWTR1 Monoclonal antibody

WWTR1 Monoclonal Antibody for IF
Cat No. CL594-66500
Clone No.2A12A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF

Synonyms

DKFZp586I1419, TAZ, WWTR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66500 targets WWTR1 in IF applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WWTR1 fusion protein Ag13330 相同性解析による交差性が予測される生物種
Full Name WW domain containing transcription regulator 1
Calculated molecular weight 44 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZV5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 594 WWTR1 antibody CL594-66500Download protocol
Standard Protocols
Click here to view our Standard Protocols