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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBC1D4 Polyclonal antibody (24667-1-AP)

    WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBC1D4 Polyclonal antibody (24667-1-AP)

    WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human heart using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TBC1D4 Polyclonal antibody (24667-1-AP)

    IHC staining of human heart using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBC1D4 Polyclonal antibody (24667-1-AP)

    IF Staining of HeLa using 24667-1-AP (same clone as 24667-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

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TBC1D4 Polyclonal antibody, PBS Only

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24667-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24667-1-PBS targets TBC1D4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D4 fusion protein Ag20327 相同性解析による交差性が予測される生物種
Full Name TBC1 domain family, member 4
Calculated molecular weight 1299 aa, 147 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC151239
Gene Symbol TBC1D4
Gene ID (NCBI) 9882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60343
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.