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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBCB Polyclonal antibody (15782-1-AP)

    WB analysis using 15782-1-AP (same clone as 15782-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15782-1-AP (TBCB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15782-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TBCB Polyclonal antibody (15782-1-AP)

    IP experiment of HeLa using 15782-1-AP (same clone as 15782-1-PBS)

    IP result of anti-TBCB (IP:15782-1-AP, 3ug; Detection:15782-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15782-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBCB Polyclonal antibody (15782-1-AP)

    IF Staining of HeLa using 15782-1-AP (same clone as 15782-1-PBS)

    Immunofluorescent analysis of Hela cells, using TBCB antibody 15782-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15782-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBCB Polyclonal antibody (15782-1-AP)

    IF Staining of HeLa using 15782-1-AP (same clone as 15782-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBCB antibody (15782-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15782-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15782-1-PBS targets TBCB in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBCB fusion protein Ag8473 相同性解析による交差性が予測される生物種
Full Name tubulin folding cofactor B
Calculated molecular weight 244 aa, 27 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC005969
Gene Symbol TBCB
Gene ID (NCBI) 1155
RRIDAB_2302986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99426
Storage Buffer PBS only
Storage ConditionsStore at -80°C.