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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBK1 Recombinant antibody (83686-3-RR)

    WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBK1 Recombinant antibody (83686-3-RR)

    WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TBK1 Recombinant antibody (83686-3-RR)

    WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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TBK1 Recombinant antibody, PBS Only

TBK1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83686-3-PBS
Clone No.240769G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83686-3-PBS targets TBK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TBK1 fusion protein Ag28894 相同性解析による交差性が予測される生物種
Full Name TANK-binding kinase 1
Observed molecular weight84 kDa
GenBank accession numberBC034950
Gene Symbol TBK1
Gene ID (NCBI) 29110
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UHD2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.