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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TBK1 Recombinant antibody

TBK1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240769G5

Cat no : 83686-3-RR

Synonyms

Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBK1 Recombinant antibody (83686-3-RR)

    WB analysis using 83686-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83686-3-RR

    Affinity and Kinetic Characterization of 83686-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83686-3-RR against Human TBK1 were performed. The affinity constant is 4.05 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83686-3-RR is TBK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TBK1 fusion protein Ag28894 相同性解析による交差性が予測される生物種
Full Name TANK-binding kinase 1
Observed molecular weight84 kDa
GenBank accession numberBC034950
Gene symbol TBK1
Gene ID (NCBI) 29110
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

Protocols

Product Specific Protocols
WB protocol for TBK1 antibody 83686-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols