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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TCEB1 Monoclonal antibody

TCEB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D6D8

Cat no : 68164-1-Ig

Synonyms

EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCEB1 Monoclonal antibody (68164-1-Ig)

    WB analysis using 68164-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68164-1-Ig (TCEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using TCEB1 Monoclonal antibody (68164-1-Ig)

    IF Staining of T-47D using 68164-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed T-47D cells using TCEB1 antibody (68164-1-Ig, Clone: 2D6D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells. HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inT-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68164-1-Ig targets TCEB1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TCEB1 fusion protein Ag22601 相同性解析による交差性が予測される生物種
Full Name transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Calculated molecular weight 112 aa, 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC013809
Gene symbol TCEB1
Gene ID (NCBI) 6921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

Protocols

Product Specific Protocols
WB protocol for TCEB1 antibody 68164-1-IgDownload protocol
IF protocol for TCEB1 antibody 68164-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A

Authors - Mingchao Mu
View Article