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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCEB1 Monoclonal antibody (68164-1-Ig)

    WB analysis using 68164-1-Ig (same clone as 68164-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68164-1-Ig (TCEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68164-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using TCEB1 Monoclonal antibody (68164-1-Ig)

    IF Staining of T-47D using 68164-1-Ig (same clone as 68164-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed T-47D cells using TCEB1 antibody (68164-1-Ig, Clone: 2D6D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68164-1-PBS in a different storage buffer formulation.

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TCEB1 Monoclonal antibody, PBS Only

TCEB1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68164-1-PBS
Clone No.2D6D8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68164-1-PBS targets TCEB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TCEB1 fusion protein Ag22601 相同性解析による交差性が予測される生物種
Full Name transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Calculated molecular weight 112 aa, 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC013809
Gene Symbol TCEB1
Gene ID (NCBI) 6921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15369
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).