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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TCF3 Monoclonal antibody

TCF3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12E5

Cat no : CL488-67140

Synonyms

bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TCF3 Monoclonal anti (CL488-67140)

    IF Staining of HepG2 using CL488-67140

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-67140 (TCF3 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67140 targets TCF3 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TCF3 fusion protein Ag15734 相同性解析による交差性が予測される生物種
Full Name transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated molecular weight 654 aa, 68 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC110580
Gene symbol TCF3
Gene ID (NCBI) 6929
RRIDAB_2883399
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TCF3 antibody CL488-67140Download protocol
Standard Protocols
Click here to view our Standard Protocols