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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TCF7L1 Polyclonal antibody (14519-1-AP)

    WB analysis of mouse brain using 14519-1-AP (same clone as 14519-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TCF7L1 Polyclonal antibody (14519-1-AP)

    WB analysis using 14519-1-AP (same clone as 14519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using TCF7L1 Polyclonal antibody (14519-1-AP)

    WB analysis of mouse brain using 14519-1-AP (same clone as 14519-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TCF7L1 Polyclonal antibody (14519-1-AP)

    IHC staining of human breast cancer using 14519-1-AP (same clone as 14519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TCF7L1 Polyclonal antibody (14519-1-AP)

    IHC staining of human breast cancer using 14519-1-AP (same clone as 14519-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TCF7L1 Polyclonal antibody (14519-1-AP)

    IF Staining of U2OS using 14519-1-AP (same clone as 14519-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TCF7L1 antibody (14519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

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TCF7L1 Polyclonal antibody, PBS Only

TCF7L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14519-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14519-1-PBS targets TCF7L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCF7L1 fusion protein Ag5983 相同性解析による交差性が予測される生物種
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Calculated molecular weight 63 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC058894
Gene Symbol TCF7L1
Gene ID (NCBI) 83439
RRIDAB_2287033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCS4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis